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Basic Cell Culture Protocols (Methods in Molecular Biology)

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Published by Humana Press .
Written in English

Subjects:

  • Cellular biology,
  • Molecular biology,
  • Science/Mathematics,
  • Life Sciences - Biology - Molecular Biology,
  • Science,
  • Tissue culture,
  • Life Sciences - Cytology,
  • Science / Cytology,
  • Laboratory Manuals,
  • Cell Culture,
  • methods

Book details:

Edition Notes

ContributionsCheryl D. Helgason (Editor), Cindy Miller (Editor)
The Physical Object
FormatHardcover
Number of Pages384
ID Numbers
Open LibraryOL8828422M
ISBN 101588292843
ISBN 109781588292841

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Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand : $ Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today. Successful hPSC culture requires the recreation of the in vivo stem cell microenvironment, or “niche”, which includes growth factors, cell-to-cell interactions and cell to matrix adhesions. Unlike many cell types, hPSCs are grown in aggregates, or colonies, which helps create this niche. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be .

Stem cell research is a rapidly expanding field with the potential to develop therapeutic agents to treat diseases as well as study disease development from early stages. The culture of human pluripotent stem cells shares many of the same protocols as standard mammalian cell culture. However, the successful culture and maintenance of human. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction.   Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, Third Edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animals cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips.   Stem cell research is a rapidly expanding field with the potential to develop therapeutic agents to treat diseases as well as study disease development from early stages. The culture of human pluripotent stem cells shares many of the same protocols as standard mammalian cell culture. However, the successful culture and maintenance of human pluripotent stem cells (hPSCs) in an undifferentiated Cited by: 1.

Basic cell culture protocols. [Cheryl D Helgason; Cindy L Miller;] This volume provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, Book\/a>, schema:CreativeWork\/a> ;. Basic cell culture protocols. [Jeffrey W Pollard; John M Walker;] Book, Internet Resource: All Authors / Contributors: Jeffrey W Pollard; John M Walker. Find more information about: ISBN: Basic cell culture --Establishment, maintenance. The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of. Most cell lines can be grown using DMEM culture media or RPMI culture media with 10% Foetal Bovine Serum (FBS), 2 mM glutamine and antibiotics can be added if required (see table below). Check which culture media and culture supplements the cell line you are using requires before starting cultures. Culture media and supplements should be sterile.